论文部分内容阅读
目的分析2011年-2016年唐山地区B型流感血凝素基因特征。方法对唐山市哨点医院流感样病例咽拭子标本进行核酸检测,阳性标本采用MDCK细胞分离培养,选取10株B型流感病毒进行HA1基因测序,分析其核苷酸序列特征及氨基酸变异情况。结果进化分析显示,唐山地区B型流感病毒分为B/Victoria系和B/Yamagata系,其中4株B/Vic系毒株为1B分支(2011年-2012年)和1A分支(2015年-2016年),6株B/Yam系毒株均属于3分支。与疫苗株B/Brisbane/60/2008相比,B/Vic系毒株氨基酸替换发生在N129S和I146V(1B分支)、I117V和N129D(1A分支)。与疫苗株B/Wisconsin/1/2010(B/Yam 3分支)相比,B/Yam毒株共有7个位点发生氨基酸替换;与疫苗株B/Massachusetts/2/2012(B/Yam 2分支)相比,有14个位点发生氨基酸替换,其中N116K、S150I、N165Y和D196N位于抗原表位上。结论 2011年-2016年唐山地区B型流感毒株发生明显变异,连续分子学监测对该地区的疫苗接种政策有重要意义。
Objective To analyze the characteristics of influenza B gene in Tangshan from 2011 to 2016. Methods Nucleic acid was detected in throat swab specimens of influenza-like cases in sentinel hospital of Tangshan City. The positive specimens were isolated and cultured with MDCK cells. 10 strains of influenza B virus were selected for HA1 gene sequencing, and their nucleotide sequences and amino acid variation were analyzed. Results Phylogenetic analysis showed that influenza B viruses were divided into B / Victoria strain and B / Yamagata strain in Tangshan area. Four strains of B / Vic strains were 1B branch (2011-2012) and 1A branch (2015-2016 Year), 6 strains of B / Yam strains belong to 3 branches. B / Vic strain amino acid substitutions occurred at N129S and I146V (1B branch), I117V and N129D (branch 1A) compared to vaccine strain B / Brisbane / 60/2008. A total of seven amino acid substitutions occurred in the B / Yam strain compared to the vaccine strain B / Wisconsin / 1/2010 (B / Yam 3 branch); and the vaccine strain B / Massachusetts / 2/2012 Compared with 14 amino acid substitutions, of which N116K, S150I, N165Y and D196N were located on the epitope. Conclusion The strains of influenza B mutated significantly in Tangshan from 2011 to 2016. Continuous molecular monitoring was of great importance to the vaccination policy in this area.