Prolonged manganese exposure induces severe deficits in lifespan,development and reproduction possib

• 作 者: XIAO Jing RUI Qi GUO Yuling CH
• 机 构: College of Life Sciences,Nanjing Normal University,Nanjing 210046,China“,”Department of Genetics and
• 出 处: 环境科学学报（英文版）
• 发表日期: 2009年6期
• 关键词: manganese toxicity prolonged exposure stress response antioxidant gene Caenorhab

We examined the possible multiple defects induced by acute and prolonged exposure to high levels of manganese (Mn) solution by monitoring the endpoints of lifespan,development,reproduction,and stress response.Our data suggest that acute exposure (6 h) to Mn did not cause severe defects of life span,development,and reproduction.Similarly,no significant defects could be found for the life span,development,and reproduction in animals exposed to a low concentration of Mn (2.5 μmol/L) for 48 h.In contrast,prolonged exposure (48-h) to high concentrations of Mn (75 and 200 μmol/L) resulted in significant defects of life span,development,and reproduction,as well as the increase of the percentage of population with hsp-16.2::gfp expression indicating the obvious induction of stress responses in exposed animals.Moreover,prolonged exposure (48-h) to high concentrations (75 and 200 μmol/L) of Mn decreased the expression levels of antioxidant genes of sod-1,sod-2,sod-3,and sod-4 compared to control.Therefore,prolonged exposure to high concentrations of Mn will induce the severe defects of life span,development,and reproduction in nematodes possibly by affecting the stress response and expression of antioxidant genes in Caenorhabditis elegans.