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目的 :为了对肝硬化门脉高压症的术式及手术时机的选择提供理论依据 ,利用门脉系置管四氯化碳注射法制备了犬肝硬化模型 ,并对肝硬化进程中肝储备功能的变化进行了研究。方法 :对成年杂种犬进行脾静脉属支置管术 ,每周 3次经管内应用 0 .8ml/ kg的1 0 % (体积分数 ) CCl4 脂肪乳溶液 ,第 2、4、6、8、1 0周末采取小块肝组织 ,测定血清生化指标 ,进行吲哚氰绿排泄试验及口服糖耐量试验 ,并测取门脉压。结果 :用药 8～ 1 0周后 ,肝硬化形成率 68.7% ,病死率 2 5%。肝储备功能在用药 8～ 1 0周间下降显著 ,吲哚氰绿清除率由0 .1 3 1± 0 .0 1 3降至 0 .0 52± 0 .0 0 9,吲哚氰绿 1 5min滞留率由 9.52± 1 .50升至 3 1 .3 0± 8.50。口服糖耐量试验 ( 1 2 0 min) 8.3± 0 .7mmol/ L升至 1 4.9± 2 .3 mmol/ L。结论 :门脉置管四氯化碳注射法制备犬肝硬化模型方法成功新颖 ,腹腔无粘连。肝硬化进展中 ,肝储备功能逐渐下降 ,总胆红素、口服糖耐量试验及吲哚氰绿排泄试验 ,对肝硬化进展程度具有较高的评价价值。
Objective: In order to provide a theoretical basis for the choice of surgical procedure and timing of cirrhosis and portal hypertension, a model of canine cirrhosis was established by injecting carbon tetrachloride into the portal vein and the hepatic reserve function The changes were studied. Methods: Adult Sprague-Dawley dogs were treated with splenic vein support and administered with 0.8% (volume fraction) CCl4 fat emulsion orally intraperitoneally three times per week. The first, second, fourth, sixth, eighth, 0 weekend to take a small piece of liver tissue, serum biochemical indicators, indocyanine green excretion test and oral glucose tolerance test, and measured portal pressure. Results: After 8 to 10 weeks of treatment, the incidence of cirrhosis was 68.7% and the mortality rate was 25%. The hepatic reserve decreased significantly during the 8-10 weeks of treatment, the clearance rate of indocyanine green decreased from 0. 131 ± 0. 01 3 to 0. 52 ± 0. 0 0 9, indocyanine green 1 5min retention rate rose from 9.52 ± 1.50 to 31.3 ± 8.50. Oral glucose tolerance test (120 min) 8.3 ± 0 .7 mmol / L rose to 1 4.9 ± 2 .3 mmol / L. Conclusion: The method of injecting carbon tetrachloride into portal vein for establishing a model of canine cirrhosis in the portal vein is successful and novel, with no adhesions in the abdominal cavity. Liver cirrhosis progress, liver function gradually declined, total bilirubin, oral glucose tolerance test and indocyanine green excretion test, the degree of progress of cirrhosis has a high evaluation value.