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目的采用正交设计试验优选四味黄连洗剂的最佳提取工艺。方法对四味黄连洗剂采用醇提取法,采用L_9(3~4)正交设计法进行试验,对提取次数、乙醇浓度、提取时间和乙醇倍数4个单因素进行试验,以黄芩苷、盐酸小檗碱、大黄素含量及干膏得率为指标,利用综合评分法对数据进行分析,优选出最佳提取工艺。结果优选出四味黄连洗剂最佳醇提工艺为处方量药材加12倍80%乙醇提取3次,每次1.5h。结论试验得到的最佳提取方法稳定可行,可应用于四味黄连洗剂的生产制备。
OBJECTIVE To optimize the extraction process of four-flavor berberine lotion by orthogonal test. Methods The alcoholic extraction method was applied to the four flavors of Huanglian Huanglian. The four factors (L_9 (3 ~ 4) orthogonal design method) were used to test the four factors, including the extraction times, the ethanol concentration, the extraction time and the alcohol multiples. Berberine, emodin content and dry extract yield as an indicator, the use of comprehensive score data analysis, the best extraction process. The results showed that the optimal alcohol extraction process for the four flavors coptis-broth was prescription extraction of medicinal materials plus 12 times 80% ethanol extraction three times for 1.5h. Conclusion The best extraction method obtained by the experiment is stable and feasible and can be applied to the production and preparation of the four-flavor Coptis lotion.