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目的探讨端粒酶活性与大肠癌及癌前病变关系．方法应用端粒酶反复扩增法（ＴＲＡＰ）对大肠癌３６例，相应的正常对照肠粘膜２０例、大肠腺瘤及息肉１５例进行端粒酶活性检测．同时检测大肠癌脱落细胞１５例端粒酶活性．结果大肠癌３６例中端粒酶活性表达３０例（８３３％），正常肠粘膜中１８例无活性（９０％），剩余２例为阳性，１５例大肠癌前病变中，腺瘤和息肉端粒酶活性分别为７７８％（７／９）和６６７％（４／６）．另外我们进一步检测１５例大肠癌患者标本脱落细胞端粒酶活性，亦发现８例阳性（５３３％），端粒酶活性与Ｄｕｋｅｓ病理分期无明显相关性（Ｐ＞００５）．结论端粒酶活性分析对大肠癌的早期诊断、预后监测及术后复发具有特殊价值
Objective To investigate the relationship between telomerase activity and colorectal cancer and precancerous lesions. Methods Thirty-six cases of colorectal cancer, 20 cases of corresponding normal intestinal mucosa, 15 cases of colorectal adenoma and polyp were examined for telomerase activity using TRAP. Simultaneous detection of telomerase activity in 15 cases of colorectal cancer exfoliated cells. Results Thirty-six cases (83. 3%) of telomerase activity were detected in 36 cases of colorectal cancer, 18 cases of normal intestinal mucosa were inactive (90%), the remaining 2 cases were positive, and 15 cases of precancerous lesions. The telomerase activity of polyps was 77.8% (7/9) and 66.7% (4/6), respectively. In addition, we further examined the telomerase activity of exfoliated cells in 15 patients with colorectal cancer and found that 8 cases were positive (53. 3%). Telomerase activity was not significantly related to the pathological stage of Dukes (P>005). Conclusion The analysis of telomerase activity has special value in the early diagnosis, prognosis monitoring and postoperative recurrence of colorectal cancer