论文部分内容阅读
目的观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)与5-氟尿嘧啶(5-Fu)联用对结肠癌细胞株HT-29体外生长增殖、凋亡的影响,评价其联用效果。方法体外培养HT-29细胞,采用磺基罗丹明B(SRB)法测定细胞的存活率,Webb系数法判断联用是否具协同作用,流式细胞FITC-AnnexinV/PI双染法检测细胞的凋亡。结果结肠癌细胞株HT-29对TRAIL不敏感(IC50>10μg·mL-1),对5-Fu敏感(IC50<10μg·mL-1);0.1、1、10μg·mL-1TRAIL分别与0.05、0.1、0.5μg·mL-15-Fu联用48h,除0.1μg·mL-1TRAIL与0.5μg·mL-15-Fu联用组外,其余联用组细胞存活率均明显低于TRAIL单用组或5-Fu单用组水平;0.1μg·mL-1TRAIL及0.5μg·mL-15-Fu单用或联用时,HT-29细胞凋亡率分别为8.6%、18.1%和30.8%。结论 5-Fu与TRAIL联用具有协同的细胞毒和细胞凋亡作用。
Objective To observe the effects of combination of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and 5-fluorouracil (5-Fu) on proliferation, apoptosis of human colon cancer cell line HT-29 in vitro and to evaluate its combination effect. Methods HT-29 cells were cultured in vitro. The viability of HT-29 cells was determined by SRB method. The synergism was determined by the Webb coefficient method. The apoptosis of HT-29 cells was detected by FITC-Annexin V / PI double staining Death. Results The colon cancer cell line HT-29 was not sensitive to TRAIL (IC50> 10μg · mL-1) and sensitive to 5-Fu (IC50 <10μg · mL-1) 0.1, 0.5μg · mL-15-Fu for 48h, the survival rates of other combined groups were significantly lower than that of TRAIL group except 0.1μg · mL-1TRAIL and 0.5μg · mL-15-Fu combined group Or 5-Fu alone. The apoptotic rates of HT-29 cells were 8.6%, 18.1% and 30.8% when 0.1μg · mL-1 TRAIL and 0.5μg · mL-15-Fu alone or in combination. Conclusions 5-Fu combined with TRAIL has synergistic cytotoxic and apoptotic effects.