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目的探讨细胞因子及趋化性细胞因子在结核患者保护性免疫中的作用。方法用ELISA方法检测结核患者及正常人外周血单个核细胞(PBMC)经重组人白细胞介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)及脂多糖(LPS)刺激后培养上清液中调节激活正常T细胞表达及分泌因子(RANTES)和巨噬细胞炎性蛋白1-α(MIP1-α)的含量。结果结核患者PBMC用TNF-α或IFN-γ加LPS刺激组RANTES的分泌量[TNF-α组(1731．86±925．60)ng／L、IFN-γ组(2 120．78±1 120．72)ng／L]明显高于单用LPS刺激组[(1 102．56±873．44)ng／L,P<0．01)]。用重组人IL-4加LPS刺激,结核患者PBMC RANTES的分泌量[(1 333．90±621．82)ng／L]与单加LPS刺激组[(1 102．56±873．44)ng／L]相比两组的差异无统计学意义(P>0．05)。正常人及结核患者的PBMC经IL-4、TNF-α、IFN-γ及LPS刺激后MIP1-α分泌量无明显变化。结论TNF-α、IFN-γ及RANTES在结核患者的保护性免疫中可能起着重要的作用。
Objective To investigate the role of cytokines and chemokines in protective immunity in patients with tuberculosis. Methods The levels of interleukin 4 (IL-4), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) γ and lipopolysaccharide (LPS), the contents of RANTES and MIP1-α in the culture supernatant were measured. Results The secretion of RANTES in PBMC of patients with tuberculosis treated with TNF-α or IFN-γ plus LPS [TNF-α group (1731.86 ± 925.60 ng / L, IFN-γ group, 120.78 ± 120 .72) ng / L] was significantly higher than that of LPS alone group [(102.56 ± 873.44) ng / L, P <0.01). The secretion of RANTES in PBMC of tuberculosis patients [(1 333.90 ± 621.82) ng / L] was significantly higher than that of LPS stimulation group [(1 102.56 ± 873.44) ng / L, / L] compared with the two groups no significant difference (P> 0.05). The secretion of MIP1-α in PBMC of normal and tuberculosis patients after IL-4, TNF-α, IFN-γ and LPS stimulation did not change significantly. Conclusion TNF-α, IFN-γ and RANTES may play an important role in the protective immunity of tuberculosis patients.