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目的检测青岛地区临床分离的大肠埃希菌株中I类整合子的分布,分析I类整合子在细菌耐药性中的作用。方法收集青岛市立医疗集团分离鉴定的非重复大肠埃希菌78株,采用K-B纸片法检测其对16种常用抗菌药物的敏感性,PCR检测I类整合子整合酶基因,分析I类整合子与细菌耐药表型的关系。结果 78株大肠埃希菌中,整合酶基因阳性菌株占53.89%,尤其在急诊科及泌尿科最为常见;I类整合子阳性菌株对复方新诺明的耐药率明显高于I类整合子阴性菌株(P<0.05)。结论青岛地区I类整合子在大肠埃希菌中分布广泛,可影响细菌的耐药性,但需进一步研究其在大肠埃希菌多重耐药性中的作用。
Objective To detect the distribution of class I integrons in clinically isolated Escherichia coli strains in Qingdao and to analyze the role of class I integron in bacterial resistance. Methods 78 strains of non-repetitive Escherichia coli isolated and identified by Qingdao Medical Group were collected. The susceptibility of 16 non-repetitive Escherichia coli strains was detected by KB disk method. The class I integronin gene was detected by PCR. Relationship with bacterial resistance phenotype. Results Among the 78 Escherichia coli isolates, 53.89% were positive for integrase gene, especially in emergency department and urology. The resistance rate of class I integron-positive strains to cotrimoxazole was significantly higher than that of class I integron Negative strains (P <0.05). Conclusion The type I integrons in Qingdao are widely distributed in Escherichia coli, which may affect the drug resistance of bacteria. However, the role of class I integrons in Escherichia coli multi-drug resistance needs to be further studied.