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盐胁迫(75mmol·L-1NaCl)下,枣树微茎段外植体成活率、生根率及枣苗的侧根条数和长度、苗高、单株重量均较对照显著减小;盐胁迫下加硅(Si)(0.75mmol·L-1KSiO3),枣苗的前述指标与对照无显著性差异。盐胁迫下,枣组织培养生根苗细胞壁提取率显著下降,细胞壁中蛋白和低分子量果胶含量显著降低,EDTA可溶性果胶和碱溶性果胶含量显著提高,半纤维素和纤维素含量降低;盐胁迫下加Si,枣苗细胞壁提取率显著提高,细胞壁主要成分的含量水平与对照相近。与盐胁迫下相比,盐胁迫下加Si,虽然枣苗果胶甲酯酶(PME)和多聚半乳糖醛酸酶(PG)活性升高未达显著性差异水平,但纤维素酶(Cx)活性显著降低,3种水解酶活性的比值均显著增大,细胞壁共价结合态H+-ATP酶活性显著升高。结果说明,提高培养基可溶性Si水平,可显著改善受盐胁迫枣苗细胞壁水解酶的活性平衡、组分含量的均衡及细胞壁内外溶质的适应性分配,从而为枣苗较好生长奠定了基础。
Under salt stress (75 mmol·L-1 NaCl), the survival rate and rooting rate of jujube explants and the number and length of lateral roots of jujube seedling, seedling height and plant weight were significantly reduced compared with the control. Under salt stress, (Si) (0.75mmol·L-1KSiO3), jujube seedlings of the aforementioned indicators and the control no significant difference. Under salt stress, the cell wall extraction rate of rooting seedlings decreased significantly, the content of protein and low molecular weight pectin in cell wall decreased significantly, the contents of EDTA soluble pectin and alkali soluble pectin increased significantly, the content of hemicellulose and cellulose decreased; Adding Si under stress, jujube seedling cell wall extraction rate was significantly increased, the main component of the cell wall content levels similar to the control. Compared with the salt stress, adding Si under salt stress, although jujube seedlings pectin methyl esterase (PME) and polygalacturonase (PG) activity did not reach significant differences, but the cellulase ( Cx) activity was significantly reduced, the ratio of three hydrolase activity were significantly increased, the cell wall covalent bound H + -ATPase activity was significantly increased. The results showed that increasing soluble Si level in medium could significantly improve the activity balance of cell wall hydrolytic enzymes, the balance of components and the adaptive distribution of solutes in cell wall, which laid the foundation for the better growth of jujube seedlings.