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目的:探讨七叶皂苷钠对一氧化碳中毒迟发性脑病大鼠模型细胞凋亡及Caspase-3表达的影响。方法:将30只大鼠随机分为正常对照组、一氧化碳中毒迟发性脑病大鼠模型对照组和七叶皂苷钠治疗组,原位末端标记法(TUNEL)检测细胞凋亡,免疫组化法检测Caspase-3的表达。结果:治疗组大鼠脑细胞凋亡率及Caspase-3表达较迟发性脑病对照组显著降低(P<0.01)。结论:七叶皂苷钠对一氧化碳中毒迟发性脑病的脑保护作用机制可能与干预脑细胞凋亡相关基因表达并减少神经细胞凋亡有关。
Objective: To investigate the effect of sodium aescinate on cell apoptosis and expression of Caspase-3 in rats with delayed encephalopathy induced by carbon monoxide poisoning. Methods: Thirty rats were randomly divided into normal control group, carbon monoxide poisoning delayed encephalopathy rat model control group and sodium aescinate treatment group. TUNEL method was used to detect the apoptosis of the rats. Immunohistochemistry Caspase-3 expression was detected. Results: The apoptotic rate and the expression of Caspase-3 in the treatment group were significantly lower than those in the control group (P <0.01). CONCLUSION: The mechanism of sodium protection of sodium aescinate on brain damage induced by carbon monoxide poisoning delayed encephalopathy may be related to the intervention of apoptosis-related gene expression and decrease of apoptosis of nerve cells.