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经过近十年的病原菌接种试验,从国内外数百份水稻种质中,筛选出了抗病水平不同的5个代表品种Lemont、武育粳3号、Jasmine85、C418和YSBR1,作为水稻纹枯病菌致病力的鉴别品种。从江苏、广东、广西、海南、云南、湖南、福建等省采集、分离的水稻纹枯病菌中,初步筛选出致病力有差异的30个菌株,于温室苗期接种上述5个品种,确定了不同致病力的5个代表菌株C30、GD-118、E67、YN-7和YN-3。将选出的品种和菌株在江苏扬州和浙江富阳进行大田成株期抗性和致病力分析,结果表明:品种的抗病性和菌株的致病力都存在极显著差异,分别属于5个和3个不同的显著性级别,均可暂时作为鉴定材料使用。据以上结果,初步建立了一套可供水稻纹枯病菌致病力检测和寄主抗病性鉴定的鉴别体系。本文还讨论了建立水稻纹枯病鉴别体系的必要性、可行性和需要进一步开展的工作。
After nearly ten years of pathogen inoculation experiments, five representative cultivars Lemont, Wuyujing 3, Jasmine85, C418 and YSBR1 with different disease resistance levels were screened out from hundreds of rice germplasm at home and abroad. Identification of pathogenic bacteria germ varieties. Thirty isolates with pathogenicity were initially screened from the isolates of Rhizoctonia solani collected from Jiangsu, Guangdong, Guangxi, Hainan, Yunnan, Hunan and Fujian provinces. The five varieties were inoculated in the greenhouse, Five representative strains C30, GD-118, E67, YN-7 and YN-3 with different pathogenicity were identified. The resistance and virulence of the selected cultivars and strains in adult plants in Yangzhou of Jiangsu Province and Fuyang of Zhejiang Province were analyzed. The results showed that there were significant differences in disease resistance and pathogenicity among the cultivars, which belonged to 5 And 3 different levels of significance, can be temporarily used as identification materials. According to the above results, a set of identification system for pathogenicity test and host disease resistance identification of Rhizoctonia solani was preliminarily established. This article also discussed the necessity, feasibility and further work needed to establish the identification system of rice sheath blight.