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在法国,消毒剂和抗生素抗病毒活性的检验必须按照标准化认证权利机构(AFNOR)的NF T 72-180和NF T72-181的条例进行。此条例规定,一种化合物与病毒悬液混合后,至少应减少4个对数级的感染单位才可以认为有抗病毒活性。检验过程中,消毒剂的细胞毒性是影响判断的主要问题,稀释病毒悬液或者经Sephedex柱凝胶过滤可以解决一部分问题;但是,不是所有消毒剂都可以被Sephedex阻隔,稀释病毒悬液往往也需要很高的病毒滴度。以往,常用膜过滤来检验消毒剂对物体表面细菌的抗菌作用,并得到AFNOR的批准。膜过滤指把清洗以后
In France, tests for the antiviral activity of disinfectants and antibiotics must be carried out in accordance with the regulations of NF T 72-180 and NF T72-181 of the Institute of Standards and Accreditation Rights (AFNOR). The regulation stipulates that a compound should be reduced to at least 4 log-level infectious units to be considered antiviral when mixed with a virus suspension. During testing, the cytotoxicity of the disinfectant is a major problem that can affect judgment. Dilution of the virus suspension or gel filtration on a Sephedex column can solve some of the problems; however, not all disinfectants can be blocked by Sephedex, and the diluted virus suspension tends to Need a high virus titer. In the past, membrane filtration was used to test the antibacterial effect of disinfectants on the bacteria on the surface of the object and was approved by AFNOR. Membrane filtration means after cleaning