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目的确定临床分离的金黄色葡萄球菌生物膜形成能力和生物膜相关基因之间的关系,为生物膜形成的机制研究提供依据。方法96孔板番红染色法检测生物膜的形成,icaAD、icaBC、sar、agr和sigB的PCR扩增,并对产物进行测序和同源性比较。结果27株金黄色葡萄球菌中共有17株形成了肉眼可见的生物膜,其中以X387和X409两株最明显;有22株扩增出icaAD和icaBC,全部菌株扩增出sar、agr和sigB。结论ica是形成金葡菌生物膜的关键基因,但ica在形成生物膜过程中需要其他基因的协同作用。
Objective To determine the relationship between biofilm-forming ability and biofilm-forming ability of clinically isolated Staphylococcus aureus and to provide evidence for the mechanism of biofilm formation. Methods Biofilm formation, icaAD, icaBC, sar, agr and sigB PCR amplification were detected by 96-well saffron staining, and the products were sequenced and compared with each other. Results A total of 17 strains of Staphylococcus aureus formed macroscopic biofilms, of which X387 and X409 were the most obvious. 22 strains of icaAD and icaBC were amplified, and all strains amplified sar, agr and sigB. Conclusion ica is a key gene in the formation of Staphylococcus aureus biofilm. However, ica requires synergy of other genes during biofilm formation.