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目的:探讨MKK4基因-1304T>G位点单核苷酸多态基因型在中国东部人群中的分布及其对食管癌易感性的影响。方法:设计以医院为基础的病例对照研究,进行食管癌患者与对照人群频数匹配,采用聚合酶链反应-限制性片段长度多态性的方法(PCR-RFLP)对571例食管癌患者和785名正常人的MKK4基因-1304T>G位点(rs3826392)进行基因分型。利用Logistic回归分析基因多态性与食管癌发病风险的关联,并校正年龄和性别。结果:MKK4基因-1304T>G位点基因多态性在病例和对照组中分布差异无统计学意义,GG基因型(OR=0.98,95%CI:0.67～1.61),TG基因型(OR=1.12,95%CI:0.90～1.43),P=0.435。结论:MKK4基因-1304T>G位点的单核苷酸多态可能与中国东部人群食管癌易感性无关。
Objective: To investigate the distribution of MKN4304T> G SNP genotypes in Eastern China population and its effect on susceptibility to esophageal cancer. Methods: A hospital-based case-control study was designed to compare the frequency of esophageal cancer patients with that of the control subjects. The frequency of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 571 esophageal cancer patients and 785 Normal human MKK4 gene -1304T> G site (rs3826392) for genotyping. Logistic regression was used to analyze the association of genetic polymorphisms with the risk of esophageal cancer and to correct for age and gender. Results: There was no significant difference in the distribution of -1304T> G locus between MKK4 and GK genotypes (OR = 0.98, 95% CI: 0.67-1.61), TG genotype (OR = 1.12, 95% CI: 0.90-1.43), P = 0.435. Conclusion: The single nucleotide polymorphism at -1304T> G site of MKK4 gene may not be associated with the susceptibility to esophageal cancer in eastern Chinese population.