,Mutation induction of Pleurotus ferulae by low-energy N+ ion implantation and characters of the sel

• 作 者: CHEN Henglei WAN Honggui ZHANG Jun ZENG Xianxian
• 机 构: College of Life Science and Pharmacy Engineering,Nanjing University of Technology,Nanjing 210009,Chi
• 出 处: 核技术（英文版）
• 发表日期: 2008年1期
• 关键词: Pleurotus ferulae N+ beam High temperature tolerance

In order to obtain Pleurotus ferulae with high temperature tolerance,mycelium mono-cells of wild type strain ACK was treated by nitrogen ion(5～30 keV,1.5×1015～1.5×1016cm-12)implantation,and mutant CGMCC1762 was selected through auxotrophy screening method,which was Lys.VB6 auxotrophy stress with high temperature.We found that during riper period the surface layer mycelium of the mutant was not aging neither grew tegument even above 30℃.The mycelium endurable temperature of the mutant was increased 70℃ compared with that of the wild type strain.The fruiting bodies growth temperature of the mutant was 16-20℃ in daytime and was 6～12℃ at night.The highest growth temperature of fruiting bodies of the mutant Was increased by 5℃ than that of original strain.Through three generation investigation,we found that the mutant CGMCC1762 was stable with high temperature tolerance.