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柿(Diospyros kaki Thunb.)优良品种一般仅开雌花且单性结实能力强,早期剔除雄性后代是提高育种效率的重要措施。在柿的近缘种君迁子(D.lotus L.)中已经筛选出一个与雄性性状紧密连锁的DlSx-AF4S标记,但其在包括栽培柿及其杂交后代性别鉴定中的有效性尚不完全明确。利用该标记对268份相关试材进行性别鉴定,并与其花性观察结果相比较。结果表明:(1)116份柿(D.kaki)种质中除‘罗田甜柿’、‘兴义水柿’、‘甘百目’、‘商城冬柿’外,其余112份试材的DlSx-AF4S标记鉴定结果与其田间性别表现一致,鉴别率96.6%;(2)11个柿(D.kaki)杂交组合共118份F1代中有51份进入成花状态,DlSx-AF4S标记检测与已知花性的46份试材一致,鉴别率90.2%;(3)DlSx-AF4S标记在‘华柿1号’ב罗田甜柿’F_1代中呈现近1︰1分离;(4)DlSx-AF4S标记在柿和君迁子之外的其他供试柿属植物中也表现出良好的通用性。以上结果表明,DlSx-AF4S标记可用于柿及其杂交后代雄性性别的早期鉴定。
The best varieties of Diospyros kaki Thunb. Are female flowers only and have strong ability of unisexuality. Early elimination of male offspring is an important measure to improve the breeding efficiency. A DlSx-AF4S marker closely linked to male traits has been screened out in D.lotus L., but its validity in sex identification, including cultivated persimmon and its offspring, is not yet known Completely clear. 268 samples were sexed using this marker and compared with their floristic observations. The results showed that: (1) In 116 germplasms of D.kaki, the remaining 112 samples except ’Luotian sweet persimmon’, ’Xingyi water persimmon’, ’Ganbai mu’ and ’Shangdong city persimmon’ The identification results of DlSx-AF4S were consistent with their gender in the field, with the identification rate of 96.6%. (2) Among 11 of 118 F1 hybrids from 11 D.kaki cultivars, 51 were in flower status. The DlSx- (4) The DlSx-AF4S marker showed nearly 1: 1 segregation in F_1 generation of ’Huaqian 1’ × ’Luotianpiushu’; (4) DlSx The -AF4S marker has also demonstrated good versatility in other persimmon plants beyond the persimmon and the Junker. The above results show that the DlSx-AF4S marker can be used for the early identification of male sex in persimmon and its progeny.