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目的:提高汉坦病毒(HV)的检出率。方法:设计了两对新的汉坦病毒(HV)通用引物,建立新的RT-PCR方法,对流行于湖北省不同地区的肾综合征出血热(HFRS)患者血清进行了检测。结果:在对166份血清中HV RNA的检测显示HTN型阳性率为80.1%(133/166),SEO型为19.9%(33/166);对不同浓度的HV RNA检测结果显示,本法最低可检测出血清中72 pg病毒基因组。结论:说明本法用于检测HFRS患者血清中HV RNA的特异性和敏感性均很高,且我国中部地区HFRS流行以HTN为优势型,这一结果不仅为HFRS的诊断提供了新的手段,也为HFRS的防治提供了新的依据。
Objective: To improve the detection rate of Hantavirus (HV). Methods: Two new universal primers of Hantavirus (HV) were designed and a new RT-PCR method was established to detect the serum of patients with hemorrhagic fever with renal syndrome (HFRS) epidemic in different areas of Hubei province. Results: The HVNV RNA of 166 serum samples showed that the positive rate of HTN was 80.1% (133/166) and that of SEO was 19.9% (33/166). The detection results of HV RNA of different concentrations showed that this method was the lowest 72 pg of the virus genome can be detected in serum. Conclusion: The specificity and sensitivity of this method for the detection of HV RNA in the serum of patients with HFRS are high, and HTN prevalence in the epidemic of HFRS in the central region of China, which not only provides a new means for the diagnosis of HFRS, It also provides a new basis for the prevention and treatment of HFRS.