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目的了解江苏省宜兴地区丙型肝炎病毒(HCV)基因型分布特征和病毒变异情况。方法对宜兴市人民医院收治的158例HCV抗体阳性患者血清进行HCV RNA检测,对阳性标本进行Simmonds分型,采用5′非编码区(5′NCR)1、2、3、1b型特异性引物进行PCR扩增,并对1b型和2 型各1株的PCR产物测序验证。分析不同性别、临床类型的丙型肝炎患者基因型分布差异。结果158份血清中,有95份为HCV RNA阳性,其中1b型80份(84.2%),2型5份(5.3%),1b/2型的混合感染5份(5.3%),不能分型的5份(5.3%),1b型的序列与GenBank J-4株的同源性为99.2%,2型与GenBank J-6株的同源性为97.7%。男性和女性在基因型总体分布上存在差异(P<0.05),并且在单一型感染和混合型感染的分布上差异亦有统计学意义,不同临床类型的丙型肝炎中HCV基因型分布未显示差异。结论江苏省宜兴地区HCV以1b型为主,男女患者HCV基因型分布差异有统计学意义,而不同临床类型的HCV基因型分布无显著差异,基本反映了本地区HCV感染的特点。
Objective To understand the distribution and variation of hepatitis C virus (HCV) genotypes in Yixing, Jiangsu Province. Methods HCV RNA was detected in 158 HCV-positive patients treated by Yixing Municipal People’s Hospital. Simmonds typing was performed on the positive samples. The specific primers of 5 ’non-coding region (5’NCR) PCR amplification was performed, and PCR products of 1 strain of type 1b and 2 were sequenced and verified. Analysis of different genders, clinical types of hepatitis C patients genotype distribution differences. Results Of 158 serum samples, 95 were positive for HCV RNA, of which 80 were type 1b (84.2%), 5 were type 2 (5.3%) and 5 were mixed type 1b / 5 (5.3% The sequence of type 1b was 99.2% homologous to GenBank J-4 and 97.7% of type 2 with GenBank J-6. There was a significant difference in the overall genotype distribution between male and female genotypes (P <0.05), and there was also a significant difference in the distribution of single genotypes and mixed genotypes among HCV genotypes in different clinical types of hepatitis C difference. Conclusion HCV genotype 1b is predominant in Yixing of Jiangsu Province. There is significant difference in the distribution of HCV genotypes between male and female patients. There is no significant difference in HCV genotype distribution between different clinical types, which basically reflects the characteristics of HCV infection in this area.