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目的观察褐藻多糖硫酸酯(FPS)对慢性肾衰竭大鼠肾间质纤维化及肾小管上皮-间充质细胞转化(EMT)的作用,并探讨该作用与肾组织转化生长因子-β1(TGF-β1)、单核细胞趋化蛋白1(MCP-1)、血小板反应蛋白1(TSP-1)和血管内皮生长因子(VEGF)表达变化的关系。方法将6周龄Wistar雄性大鼠随机分为3组,即正常对照组(n=15),进食普通饲料;CRF模型组(n=24),进食含0.75%腺嘌呤的饲料;CRF+FPS组(n=24),进食含0.75%腺嘌呤饲料的同时,每天给予FPS200mg/kg灌胃。分别于第2、4、6周末处死大鼠,留取肾组织。采用免疫组化方法检测肾组织肌动蛋白α(α-SMA)的表达,分别采用免疫组化方法和逆转录酶PCR法(RT-PCR)检测肾组织TGF-β1、MCP-1、TSP-1及VEGF的蛋白和mRNA表达情况。结果第2、4、6周时CRF+FPS组大鼠肾组织α-SMA蛋白表达均明显低于同期CRF模型组(P<0.05)。CRF+FPS组大鼠肾组织第2、4周时TGF-β1、MCP-1、TSP-1表达明显低于同期CRF模型组(P<0.05),而第2周时TGF-β1、MCP-1、TSP-1的mRNA表达明显低于同期CRF模型组(P<0.05),第4周时MCP-1的mRNA表达明显低于同期CRF模型组(P<0.05)。CRF+FPS组第2、4周时肾组织VEGF蛋白和mRNA表达明显高于同期CRF模型组(P<0.05)。结论FPS对慢性肾衰竭大鼠肾小管上皮-间充质细胞转化具有抑制作用,该作用与肾组织TGF-β1、MCP-1及TSP-1的表达下调和VEGF表达上调有关,这可能部分解释FPS的肾保护作用机制。
Objective To observe the effect of fucoidan on the renal tubulointerstitial fibrosis and renal tubular epithelial-mesenchymal transition (EMT) in rats with chronic renal failure and to explore the effect of fucoidan on the expression of transforming growth factor-β1 β1, MCP-1, TSP-1 and VEGF in gastric mucosa. Methods Six-week-old Wistar male rats were randomly divided into three groups: normal control group (n = 15) fed normal diet; CRF model group (n = 24) fed with 0.75% adenine diet; CRF + FPS Group (n = 24). Fed with FPS containing 200mg / kg gavage daily while consuming 0.75% adenine feed. Rats were sacrificed at the end of the 2nd, 4th, 6th week respectively, and the kidneys were collected. The expression of α-SMA in renal tissue was detected by immunohistochemical method. The expressions of TGF-β1, MCP-1 and TSP-1 in renal tissue were detected by immunohistochemistry and reverse transcriptase polymerase chain reaction (RT- 1 and VEGF protein and mRNA expression. Results The expression of α-SMA protein in CRF + FPS group was significantly lower than that in CRF model group (P <0.05) at 2, 4 and 6 weeks. The expression of TGF-β1, MCP-1 and TSP-1 in renal tissue of CRF + FPS group was significantly lower than that of CRF model group at the second and fourth week (P <0.05) 1, the mRNA expression of TSP-1 was significantly lower than the CRF model group (P <0.05), the mRNA expression of MCP-1 in the 4th week was significantly lower than that of the CRF model group (P <0.05). The expressions of VEGF protein and mRNA in renal tissues of CRF + FPS group were significantly higher than those of CRF model group (P <0.05). Conclusions FPS can inhibit renal tubular epithelial-mesenchymal transition in rats with chronic renal failure, which may be partially explained by down-regulation of TGF-β1, MCP-1, TSP-1 and up-regulation of VEGF expression FPS mechanism of renal protection.