论文部分内容阅读
目的探讨内陆地区自然环境中战备消毒包内细菌来源。方法根据战备消毒包内蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种筛选战备仓库空气同种细菌不同菌株,提取基因组DNA,以其为模板,对16S rRNA和23SrRNA的转录间隔区(internal transcribed spacer,ITS)序列进行PCR扩增,将扩增产物回收测序,将测得的基因序列用Blast进行比对。结果蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种DNA PCR扩增产物分别在850、1 200、1 000 bp左右显现单一特异性条带;战备消毒包与空气同种细菌不同菌株之间的ITS序列相似性为98%～99%。结论内陆地区自然环境中战备消毒包内细菌来源于空气细菌。
Objective To explore the origin of bacteria in war preparation disinfection packets in the natural environment of inland areas. Methods The strains of Bacillus cereus, Micrococcus luteus and Staphylococcus aureus were used to screen different bacterial strains of the same bacterium in the war preparedness warehouse. Genomic DNA was extracted and used as a template for the transcription of 16S rRNA and 23S rRNA The PCR products were amplified from the internal transcribed spacer (ITS) sequences, and the amplified products were sequenced. The sequences of the tested genes were aligned with Blast. Results DNA PCR amplification products of Bacillus cereus, Micrococcus luteus and Staphylococcus aureus subsp. Ureaplasma showed a single specific band at about 850, 1 200 and 1 000 bp, respectively; ITS sequences between different strains of similarities of 98% to 99%. Conclusion The bacteriological bacteriostasis in the natural environment in inland areas originated from airborne bacteria.