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[目的]探讨荧光原位杂交(FISH)技术在检测p53基因缺失中的应用,并评估其应用于肺腺癌诊断的可行性及临床应用前景。[方法]采用FISH技术在基因水平上检测30例肺腺癌及20例良性病变的p53基因缺失情况,比较两组间有无统计学差异,同时建立阳性检测的阈值,比较两组间p53基因缺失的阳性率。采用免疫组化检测30例肺腺癌p53蛋白表达情况,并分析肺腺癌p53基因缺失和p53蛋白阳性表达之间有无统计学意义。[结果]30例肺腺癌p53基因缺失率为(38.3%±8.9%),20例良性病变对照组为(13.3%±2.4%),两组间有显著统计学差异(P<0.01)。p53蛋白阳性组与阴性组间的p53基因缺失率无统计学意义(P>0.05)。[结论]p53基因缺失的高表达在肺腺癌的发生中可能起重要作用,用FISH技术检测p53基因缺失率可能对肺腺癌诊断和治疗提供更加准确的依据。
[Objective] To investigate the application of fluorescence in situ hybridization (FISH) in the detection of p53 gene deletion and to evaluate its feasibility and clinical application in the diagnosis of lung adenocarcinoma. [Method] FISH was used to detect the deletion of p53 gene in 30 cases of lung adenocarcinoma and 20 cases of benign lesions at gene level. The differences of p53 gene between the two groups were compared. At the same time, the threshold of positive detection was established. The p53 gene Missing positive rate. Immunohistochemistry was used to detect the expression of p53 protein in 30 cases of lung adenocarcinoma and to analyze whether p53 gene deletion and p53 protein expression in lung adenocarcinoma were statistically significant. [Results] The p53 gene deletion rate was (38.3% ± 8.9%) in 30 cases of lung adenocarcinoma and (13.3% ± 2.4%) in 20 cases of benign lesions. There was significant difference between the two groups (P <0.01). There was no significant difference in p53 gene deletion between p53 positive and negative groups (P> 0.05). [Conclusion] The high expression of p53 gene deletion may play an important role in the development of lung adenocarcinoma. Detecting the loss of p53 gene by FISH may provide a more accurate basis for the diagnosis and treatment of lung adenocarcinoma.