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目的:本实验通过中药对肝星状细胞和肝组织蛋白质组的干预实验,从蛋白质组学角度进一步揭示肝纤维化的发病机制和中药的药理作用,为新药研制提供理论依据。方法:制备正常大鼠肝星状细胞、加入血小板衍生生长因子的肝星状细胞及加入肝复康药物血清和血小板衍生生长因子的肝星状细胞,分别设定为正常对照组、模型组及治疗组;同时分别将注射二甲基亚硝胺、肝复康的大鼠设定为模型组及治疗组,并将各组大鼠肝脏取出。分别提取以上各组的总蛋白质并进行等电聚焦电泳,随后进行SDS-PAGE电泳。凝胶染色后比对蛋白质斑点,找出上调和下调的蛋白质。结果:随着治疗时间的不断延长,HSC的蛋白质表达在不同时间有了不同变化。大鼠造模4周后,模型组与治疗组肝组织总蛋白质图谱存在着一定差异,部分蛋白质在两组中表现出不同的丰度。结论:肝纤维化的发生与多种蛋白质的作用有关。中药肝复康治疗肝纤维化是通过调节多个蛋白质表达产生的。
OBJECTIVE: In this experiment, through the intervention experiment of traditional Chinese medicine on proteomics of hepatic stellate cells and liver tissue, the pathogenesis of hepatic fibrosis and the pharmacological action of traditional Chinese medicine were further revealed from the perspective of proteomics, providing a theoretical basis for the development of new drugs. Methods: Hepatic stellate cells (HSCs) were prepared from normal rat hepatic stellate cells (HSCs) and hepatic stellate cells (PLGFs) and hepatic stellate cells (PLGFs) were added into the hepatic stellate cells. At the same time, the rats injected with dimethylnitrosamine and Ganfukang were respectively set as the model group and the treatment group, and the liver of each group was removed. The total proteins in each of the above groups were extracted and subjected to isoelectric focusing electrophoresis, followed by SDS-PAGE electrophoresis. Protein spots were spotted after gel staining to identify up- and down-regulated proteins. Results: With the continuous extension of treatment time, protein expression of HSC changed at different times. Four weeks after modeling in rats, there was a certain difference in the total protein profiles between the model group and the treatment group, and some proteins showed different abundance in the two groups. Conclusion: The occurrence of liver fibrosis is related to the function of many proteins. Traditional Chinese medicine liver rehabilitation treatment of liver fibrosis by regulating multiple protein expression.