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Objective: To explore the possible underlying mechanism by investigating the effect of electroacupuncture (EA) treatment on the primary motor cortex and striatum in a unilateral 6-hydroxydopamine (6-OHDA) induced rat Parkinson’s disease (PD) model.Methods: Male Sprague-Dawley rats were randomly divided into sham group (n=16),model group (n=14),and EA group (n=14).EA stimulation at Dazhui (GV 14) and Baihui (GV20) was applied to PD rats in the EA group for 4 weeks.Behavioral tests were conducted to evaluate the effectiveness of EA treatment.Metabolites were detected by 7.0 T proton nuclear magnetic resonance.Results: Following 4 weeks of EA treatment in PD model rats,the abnormal behavioral impairment induced by 6-OHDA was alleviated.In monitoring changes in metabolic activity,ratios of myoinositol/creatine (Cr) and N-acetyl aspartate (NAA)/Cr in the primary motor cortex were significantly lower at the injected side than the non-injected side in PD rats (P=0.024 and 0.020).The ratios of glutamate + glutamine (Glx)/Cr and NAA/Cr in the striatum were higher and lower,respectively,at the injected side than the non-injected side (P=0.046 and 0.008).EA treatment restored the balance of metabolic activity in the primary motor cortex and striatum.In addition,the taurine/Cr ratio and Glx/Cr ratio were elevated in the striatum of PD model rats compared to sham-lesioned rats (P=0.026 and 0.000).EA treatment alleviated the excessive glutamatergic transmission by down-regulating the striatal Glx/Cr ratio (P=0.001).The Glx/Cr ratio was negatively correlated with floor plane spontaneous locomotion in PD rats (P=0.027 and P=0.0007).Conclusions: EA treatment is able to normalize the metabolic balance in the primary motor cortex and striatum of PD rats,which may contribute to its therapeutic effect on motor deficits.The striatal Glx/Cr ratio may serve as a potential indicator of PD and a therapeutic target of EA treatment.