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Objective To observe the intestinal mucosal injury and the change of TNF-αcontent in rabbits with hemorrhagic shock/reperfusion(HS-R)and the effects of ganoderma Lcidum polysaccharide(GLP)on them.Methods 30rabbits were made into hemorrhagic shock,then reperfused with different liquids.These rabbits were divided by random number table into three groups:sham operation group(Sham group),reperfusion with normal saline group(NS group),reperfusion with 1%GLP group(LS group).Bacterial translocation of blood and TNF-αcontent in serum were respectively observed at the time before shock,40 min after shock,40 min and 90 min after.TNF-αcontent in intestinal mucosa and the degree of intestinal mucosal injury were examined at 90 min post-resuscitation.Results 1 With the extension of reperfusion time,the positive rate of blood bacteria increased gradually in NS group,which was significantly higher than that of Sham group and LS group(P<0.05),meanwhile the degree of intestinal mucosal injury in NS group was more severe than that of Sham group and LS group too(P<0.05).2TNF-αcontent in serum of NS group and LS group were increased obviously compared with that before shock and in Sham group(P<0.05).TNF-αcontent in serum was further increased after reperfusion with NS,which was distinctly higher than that in LS group.TNF-αcontent in intestinal mucosa of NS group was significantly higher than that in LS group and Sham group too(P<0.05).Conclusion GLP can protect intestinal mucosa against HS-R injury,and its effects may relate with the change of TNF-αin hemorrhagic shock rabbits.
Objective To observe the intestinal mucosal injury and the change of TNF-αcontent in rabbits with hemorrhagic shock / reperfusion (HS-R) and the effects of ganoderma Lcidum polysaccharide (GLP) on them. Methods 30rabbits were made into hemorrhagic shock, then reperfused with different liquids.These rabbits were divided by random number table into three groups: sham operation group (Sham group), reperfusion with normal saline group (NS group), reperfusion with 1% GLP group (LS group). Bacterial translocation of blood and TNF -αcontent in serum were observed at the time before shock, 40 min after shock, 40 min and 90 min after.TNF-αcontent in intestinal mucosa and the degree of intestinal mucosal injury were examined at 90 min post-resuscitation. Results 1 With the extension of reperfusion time, the positive rate of blood bacteria increased gradually in NS group, which was significantly higher than that of Sham group and LS group (P <0.05), meanwhile the degree of intestinal mucosal injury in NS group was more severe than that of Sham group and LS group too (P <0.05) .2 TNF-αcontent in serum of NS group and LS group were more likely with that before shock and in Sham group (P <0.05) .TNF- αcontent in serum was further increased after reperfusion with NS, which was distinctly higher than that in LS group.TNF-αcontent in intestinal mucosa of NS group was significantly higher than that in LS group and Sham group too (P <0.05) .Conclusion GLP can protect intestinal mucosa against HS-R injury, and its effects may relate with the change of TNF-αin hemorrhagic shock rabbits.