Detection of Ratoon Stunting Disease in Virus-free Seed- cane via Real-time Fluorescence Quantitativ

• 作 者: Ming DAN Song LI Kunxing YU Li
• 机 构: GuangxiSugarcaneResearchInstitute
• 出 处: 农业生物技术：英文版
• 发表日期: 2012年5期
• 关键词: 实时荧光定量PCR 甘蔗宿根矮化病 技术检测 脱毒种薯 组织培养 种苗生产 RSD PCR方法 Sugarcane Virus-free seedcane
• 基金项目: Supported by Special Funds for Basic Scientific Research of Guangxi Sugarcane Research Institute （ G2009006, G2010006, G2009015 ） , Sci-tech Research and Development Program of Guangxi Academy of Agri

This study was to develop the real-time fluorescence quantitative PCR technique for detecting the ratoon stunting disease (RSD) in virus-free seedcane seedlings. Healthy tissue culture seedlings were obtained from six plants of sugarcane ROC22, which had