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为了研究两种电泳方法在新陆早系列材料SSR分析不同阶段使用效果的可行性,本研究利用2.5%琼脂糖凝胶和6%聚丙烯酰胺凝胶对新陆早系列材料SSR扩增结果进行检测。结果显示,两种电泳方式都能够得到较为清晰的扩增条带,但在获得的条带总数及多态性比率上有所差异。琼脂糖凝胶电泳共检测出30条条带,其中有18条为多态性条带,多态性比率为60%;而在聚丙烯酰胺电泳方法共检测出50条条带,多态性条带为33条,多态性比率为66%。基于两种电泳方法所获得的SSR标记位点,利用SPSS和PopGen32软件计算遗传相似系数,并构建遗传关系聚类图。分析结果显示,两种电泳方法所获得的聚类结果大致相同,在个别品种的分析结果上存在着一定程度上的差异。其中,聚丙烯酰胺凝胶获得的分析结果与实际遗传背景拟合性较好,所以,在最终的SSR标记分析过程中采用6%的聚丙烯酰胺凝胶进行分析效果更好,而琼脂糖凝胶电泳更加适合早期SSR标记引物的大量筛选。为新陆早系列材料SSR标记快速、准确分析提供了研究方法与技术支持。
In order to study the feasibility of using two electrophoresis methods in different stages of SSR analysis of Xinluzao series materials, the results of SSR amplification of Xinluzao series with 2.5% agarose gel and 6% polyacrylamide gel Detection. The results showed that both electrophoresis methods can be more clearly amplified bands, but in the total number of bands and polymorphisms obtained rate differences. Seventy bands were detected by agarose gel electrophoresis, of which 18 were polymorphic bands with a polymorphism ratio of 60%. However, 50 bands and polymorphisms were detected by polyacrylamide electrophoresis The number of bands was 33 and the percentage of polymorphisms was 66%. Based on the SSR marker loci obtained by the two electrophoresis methods, the genetic similarity coefficients were calculated by SPSS and PopGen32 software, and the genetic relationship clustering map was constructed. The analysis results show that the clustering results obtained by the two electrophoresis methods are roughly the same, and there is a certain degree of difference in the analysis results of individual varieties. Among them, polyacrylamide gels obtained good analytical results and the actual genetic background good fit, so, in the final SSR marker analysis using 6% polyacrylamide gel analysis is better, and agarose condensate Gel electrophoresis is more suitable for large selection of early SSR marker primers. It provided research methods and technical support for rapid and accurate analysis of SSR markers of Xinluo Early Series materials.