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在比较了多种碱性染料后选乙基紫制成了乙基紫——鹅脱氧胆酸溶于辛醇的液膜电极和该活性物与季铵——胆酸按3∶1混合制成的混合液膜电极。前者在1×10~(-4)—5×10~(-3)mol·L~(-1)间对鹅脱氧胆酸根有Nernst响应,后者线性下限可扩展到3×10~(-5)mol·.L~(-1)。灵敏度有这样高的同类电极尚无报导.电极对脱氧胆酸根、甘氨鹅脱氧胆酸根等胆汁酸根也有响应,亦可单独测量之。将电极应用于ca~(2+)与鹅脱氧胆酸结合情况的测定及胆汁酸盐CMC的测量等方面,获得了一定的成功,但电极的PVC化试验结果不理想.
Ethyl violet-chenodeoxycholic acid was dissolved in the octanol liquid membrane electrode and the active compound was mixed with quaternary ammonium-cholic acid at a ratio of 3: 1 The mixed liquid membrane electrode. The former has a Nernst response to chenodeoxycholate at 1 × 10 -4 -4 × 10 -3 mol·L -1 with a linear lower limit of 3 × 10 ~ 5) mol ··L -1. Sensitivity with such a high similar electrode has not been reported.Electrode on deoxycholic acid, glycochenodeoxycholate and other bile acid also respond, can also be measured separately. The electrode has been used successfully in the determination of the binding state of ca 2+ with chenodeoxycholic acid and the measurement of bile salts CMC. However, the results of PVC test are not satisfactory.