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采用PCR方法体外扩增变链菌NG8gtfB基因531bpDNA片断,纯化后构建了含该片段的克隆并进行了DNA序列测定,为从分子水平揭示变链菌的致龋机制提供信息。
The 531bp DNA fragment of Streptomyces sp. NG8gtfB gene was amplified by PCR in vitro. After purification, the cloned fragment was cloned and sequenced. This study will provide information for revealing the mutagenic mechanism of Streptococcus mutans at the molecular level.