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为研究人内淋巴囊(ES)交感神经支配,取听神经瘤手术切除的人ES标本,用改良乙醛酸组化技术,使组织内单胺类物质发出明亮的荧光,观察ES内肾上腺素能神经元和轴索曲张体。具体方法如下:在经迷路听神经瘤切除术中,用金刚钻磨去ES后外侧表面的骨质,使其充分暴露至内淋巴管并切断,从前庭小管的前内侧壁上摘取ES。标本经液氮速冻后,沿纵向冰冻切
In order to investigate the sympathetic innervation of human lymphatic sac (ES), human ES specimens surgically removed from the neuromas were treated by modified glyoxylate staining to make the monoamines brightly fluoresce in the tissues. The adrenergic energy Neurons and axons. The specific method is as follows: In the lost acoustic neuroma resection, with diamond grinding to the outside surface of the bone after ES, so that it is fully exposed to the endolymphatic and cut off, from the vestibular canal before the inner wall of the ES removed. Frozen specimens after liquid nitrogen, along the longitudinal frozen cut