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AIM: To study the effect of genistein (GST) on rabbit heart ischemia/reperfusion (I/R) injury. METHODS: Rabbit heart I/R injury was induced by occluding the left anterior descending coronary artery for 45 min and reperfusing for 180 min. GST (1.0 mg/kg) was intravenously injected 5 min before heart ischemia. Hemodynamic data, infarct size, and cardiomyocytic apoptosis were measured. The pathologic changes of I/R myocardium were observed. RESULTS: During the I/R, heart rate, mean arterial blood pressure, myocardial oxygen consumption, left ventricu- lar (LV) –dp/dtmax and +dp/dtmax were decreased progressively. The infarct size was occupied 60.23 %±3.97 % (% of area at risk) in vehicle +I/R group while GST reduced the infarct size to 39.62 %±4.30 % (P<0.01). DNA ladder patter in myocardium was revealed by agarose gel electrophoresis in vehicle +I/R group while was not found in GST+I/R group. Apoptotic cardiomyocytes were sparse within ischemic myocardium at risk in GST+I/R group as compared with that in vehicle +I/R group (TUNEL stain). Apoptosis rate in ischemic myocardium from vehicle +I/R and GST+I/R groups detected by flow cytometry were 15.33 %±1.31 % and 3.88 %±0.33 %, respectively. Fas and Bax protein expressions in ischemic myocardium of vehicle +I/R group were higher than that in GST+I/R group (P<0.01). Bcl-2/Bax ratio in vehicle +I/R group was lower than that in nonischemic myocar- dium (P<0.01), while in GST+I/R group, the Bcl-2/Bax ratio was higher than that in vehicle +I/R group (P<0.01). CONCLUSION: GST reduced infarct size and apoptosis of myocytes in I/R rabbit heart.
AIM: To study the effect of genistein (GST) on rabbit heart ischemia / reperfusion (I / R) injury. METHODS: Rabbit heart I / R injury was induced by occluding the left anterior descending coronary artery for 45 min and reperfusing for 180 min The pathologic changes of I / R myocardium were observed. RESULTS: During the I / R, heart rate , mean arterial blood pressure, myocardial oxygen consumption, left ventricu-lar (LV) -dp / dtmax and + dp / dtmax were decreased progressively. The infarct size was occupied 60.23% ± 3.97% (% of area at risk) in vehicle + I / R group while GST reduced the infarct size to 39.62% ± 4.30% (P <0.01). DNA ladder patter in myocardium was revealed by agarose gel electrophoresis in vehicle + I / R group was not found in GST + I / R group. Apoptotic cardiomyocytes were sparse within ischemic myocardium at risk in GST + I / R group as compared with that in vehicle + I / R group (TUNEL stain). Apoptosis rate in ischemic myocardium from vehicle + I / R and GST + I / R groups detected by flow cytometry were 15.33% ± 1.31% and 3.88% ± 0.33% Bcl-2 / Bax ratio in vehicle + I / R group was higher than that in GST + I / R group (P <0.01) that in nonischemic myocar-dium (P <0.01), while in GST + I / R group, the Bcl-2 / Bax ratio was higher than that in vehicle + I / R group size and apoptosis of myocytes in I / R rabbit heart.