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目的研究三黄泻心汤(简称泻心汤)对全脑缺血再灌注(Ischemia Reperfusion,I/R)损伤大鼠的神经保护作用。方法采用两侧颈总动脉夹闭结合低血压方法制备I/R损伤大鼠模型,于I/R 6h及48h分别对大鼠进行神经功能学评分,48h后牺牲大鼠,测定大鼠脑组织中NO含量,TNOs、i NOs活力,及i NOs mRNA表达。结果泻心汤(8.74 g·kg-1·d-1)能显著改善I/R损伤48h时大鼠的行为,明显减少I/R大鼠脑内NO含量,降低TNOs、i NOs活力,抑制i NOs mRNA的表达。结论泻心汤可通过抑制I/R大鼠脑内i NOs mRNA表达,降低TNOs、i NOs活力,进而发挥对I/R损伤大鼠的神经保护作用。
Objective To study the neuroprotective effects of Sanhuang Xiexin Decoction (Xinshintang Decoction) on rats with Ischemia Reperfusion (I / R) injury. Methods I / R injury rat model was prepared by bilateral common carotid artery occlusion and hypotension. Neurological function was assessed at I / R 6h and 48h respectively. After 48h, rats were sacrificed and rat brain tissue was measured NO content, TNOs, i NOs activity, and i NOs mRNA expression. Results Xiexin Decoction (8.74 g · kg-1 · d-1) can significantly improve the behavior of rats at 48 h after I / R injury, significantly reduce the content of NO in the brain of I / R rats, decrease the activity of TNOs and i NOs, i NOs mRNA expression. Conclusion XieXinTang can inhibit i / R rat brain iNOS mRNA expression, reduce TNOs, i NOs vitality, and then play a protective effect of I / R injury in rats.