STUDIES ON 4-OXALYSINE-RESISTANT MUTANT CELL LINE AND ITS REGENERATED PLANT TISSUE FROM NICOTIANA TA

• 作 者: 罗士韦 何卓培 徐淑平 徐竹筠 李文安
• 机 构: Laboratory of Cell Physiology, Shanghai Institute of Plant Physiology, Academia Sinica,Laboratory of
• 出 处: Science in China,Ser.B
• 发表日期: 1983年01期
• 关键词: mutant parent callus tobacco lysine accumulated regenerated peroxidase successiv

A 4-oxalysine-resistant cell line from tobacco callus was selected and established through successive treatments with 1-4-oxalysine(OL, 1g/1), 2% EMS and then OL(4g/1). The mutant callus was not cross resistant to Eth and 5MT and accumulated twice amount of lysine as that of the parent. Mutant pleats were regenerated from the mutant cells. The mutant plants flowered and set seeds within the culture flasks. A secondary mutant cell line was induced from the mutant plant. The eL-resistance, lysine content and peroxidase isoenzyme of both the mutant cell and secondary cell line were examined and confirmed that all these traits, which were different from those of the parent line, were transmitted through the mutant cell line to its secondary callus. Experiment on sexual transmission is under way.

A 4-oxalysine-resistant cell line from tobacco callus was selected and established through successive treatments with 1-4-oxalysine (OL, 1 g / 1), 2% EMS and then OL resistant to Eth and 5MT and accumulated twice amount of lysine as that of the parent. Mutant pleats were regenerated from the mutant cells. The mutant plants flowered and set seeds within the culture flasks. A secondary mutant cell line was induced from the mutant plant. The eL-resistance, lysine content and peroxidase isoenzyme of both the mutant cell and secondary cell line were examined and confirmed that all of these traits, which were different from those of the parent line, were transmitted through the mutant cell line to its secondary callus. Experiment on sexual transmission is under way.