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目的探讨柚皮素或柚皮苷对人子宫颈癌Hela细胞增殖及凋亡的影响。方法柚皮素或柚皮苷(0.250、0.125、0.063g/L)作用于人子宫颈癌Hela细胞后,MTT比色法检测Hela细胞增殖活性和半数抑制浓度(half maximal inhibitory concentration,IC50);流式细胞术检测柚皮素对Hela细胞凋亡的影响。结果柚皮素能抑制Hela细胞增殖,且呈剂量和时间依赖性,24、48和72hIC50值分别为0.24、0.11和0.07g/L;柚皮苷无抑制Hela细胞增殖的作用。柚皮素组(0.22g/L、0.11g/L、0.05g/L,作用48h)人子宫颈癌Hela细胞凋亡率高于对照组,差异有统计学意义(P=0.000239681,P=0.00012746,P=5.09328E-05,P<0.01)。结论柚皮中黄酮类提取物具有抑制人子宫颈癌Hela细胞增殖作用,其中柚皮素作用明显,部分作用机制与诱导肿瘤细胞凋亡有关。柚皮苷无抗人子宫颈癌作用。
Objective To investigate the effects of naringenin or naringin on the proliferation and apoptosis of human cervical carcinoma Hela cells. Methods Naringin or naringin (0.250, 0.125 and 0.063 g / L) was applied to human cervical carcinoma Hela cells. MTT assay was used to detect the proliferation activity and half maximal inhibitory concentration (IC50) of Hela cells. Effect of Naringenin on Hela Cell Apoptosis by Flow Cytometry. Results Naringenin could inhibit the proliferation of Hela cells in a dose and time dependent manner. The IC50 values of 24, 48 and 72 h were 0.24, 0.11 and 0.07 g / L, respectively. Naringin did not inhibit the proliferation of Hela cells. The apoptosis rate of human cervical cancer Hela cells in naringenin group (0.22g / L, 0.11g / L, 0.05g / L, 48h) was significantly higher than that in control group (P = 0.000239681, P = 0.00012746 , P = 5.09328E-05, P <0.01). Conclusion Flavonoids from pomelo peel extract can inhibit the proliferation of human cervical carcinoma Hela cells. Naringenin has obvious effect and part of the mechanism is related to the induction of tumor cell apoptosis. Naringin without anti-human cervical cancer effect.