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高效液相色谱法适用的范围很广,是非常重要的分析方法之一,它在医药学及生命科学中已成为一种主要的分离检测手段。与分离方法迅速发展的同时,检测技术的不断进步也是高效液相色谱技术得到广泛应用的原因之一。近年来高效液相色谱检测技术从最常用的紫外可见分光光度检测器和差示折光检测器开始,已经发展了诸如能够实时定性和定量的二极管阵列紫外可见分光光度检测器,能够快速扫描和光谱分辨率更高的色散型紫外可见分光光度检测器以及适用于生物化学的电化学检测器等等。各种联用检测手段如与质谱联用的热喷雾和粒子束接口等已日趋成熟,与傅里叶变换红外光谱和拉曼光谱的联用技术亦在发展中。各种检测器的灵敏度、线性范围、稳定性和重现性等主要指标日益提高。许多新的检测手段已为科技工作者所熟悉和使用。目前比较引人注目和发展较快的为手性化合物
High performance liquid chromatography has a wide range of applications and is one of the most important analytical methods. It has become a major separation and detection tool in pharmaceutical and life sciences. With the rapid development of the separation method, the continuous improvement of the detection technology is one of the reasons why the high performance liquid chromatography technology is widely used. In recent years, high performance liquid chromatography detection technology from the most commonly used UV visible spectrophotometric detector and differential refractive index detector has been developed, such as real-time qualitative and quantitative diode array UV-visible spectrophotometric detector capable of rapid scanning and spectroscopy Resolution higher dispersion of UV-visible spectrophotometric detector and for biochemical electrochemical detector and so on. A variety of combined detection methods such as thermal spray and particle beam interface with mass spectrometry have become more mature, and the combination of Fourier transform infrared spectroscopy and Raman spectroscopy is under development. A variety of detector sensitivity, linear range, stability and reproducibility and other major indicators of increasing. Many new tests have become familiar to and used by scientists. Currently more attractive and rapid development for the chiral compounds