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有文献报道,细胞核仁和核仁嗜银区(AgNoR-蛋白)出现相应的变化,可作为肿瘤的标志;核仁数量和成分的变化,是评定瘤细胞恶变和分化程度的标准之一。进行膀胱粘膜活检40例,其中慢性增生性膀胱炎5例,非侵袭性高分化移行细胞癌15例,侵袭性中分化和低分化移行细胞癌15例。膀胱粘膜无病变5例(正常对照组)。组织切片用硝酸银浸染法,使核仁AgNoR-蛋白区显示嗜银颗粒,用光镜(900倍油浸镜)检查200个细胞,计算嗜银颗粒平均数。同时用电镜检查AgNoR-蛋白区,计算核仁系数(有核仁胞核和无核仁胞核的比例)和嗜银颗粒系数(嗜银颗粒占据核仁面积的比例)。结果光镜检查,嗜银颗粒平均数:正常为1.15±0.1,增生性膀胱炎2.1±0.5,非侵袭性癌2.87±0.17,侵袭性癌
It has been reported in the literature that nucleolar and nucleolar argyrophilic argyrophilic zone (AgNoR-protein) appear corresponding changes, can be used as a tumor marker; nucleolar amount and composition changes, is to assess the extent of malignant tumor cells and differentiation criteria. 40 cases of bladder mucosa biopsy, including 5 cases of chronic proliferative cystitis, non-invasive well-differentiated transitional cell carcinoma in 15 cases, invasive differentiation and poorly differentiated transitional cell carcinoma in 15 cases. 5 cases of bladder mucosa without disease (normal control group). Tissue sections were disseminated with silver nitrate so that the nucleolar AgNoR-protein region showed an argyrophilic granule. 200 cells were examined with a light microscope (900 times immersion mirror) to calculate the average number of argyrophilic grains. Meanwhile, the AgNoR-protein region was examined by electron microscopy to calculate the nucleolar coefficient (the ratio of nucleated nuclei and nucleated nuclei) and the argyrophilic granule coefficient (the proportion of argyrophilic granule occupied nucleolus area). Results Light microscopy, average number of argyrophilic granules: normal 1.15 ± 0.1, proliferative cystitis 2.1 ± 0.5, noninvasive cancer 2.87 ± 0.17, invasive cancer