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目的:制备PP1脂质体,筛选最优处方。方法:用薄膜水化法制备PP1脂质体,以高效液相色谱法(HPLC)测定PP1脂质体的包封率,以包封率为主要指标,选取药脂比、胆固醇磷脂比、温度和水化时间为因素,用正交试验筛选最优配方。结果:用薄膜水法制备的PP1脂质体的最优处方的组成为:药脂比为1:10,胆固醇与二棕榈酰磷脂酰胆碱(DPPC)比为1:8,温度为40℃,水化时间为3 min。平均包封率为(63.27±3.32)%。PP1脂质体的平均粒径为(157.73±9.74)nm,Zeta电位为(-4.74±0.44)m V。结论:用薄膜水化法制备出的PP1脂质体包封率高,形态和粒径均匀,重现性好,为研究其在眼部的缓释作用奠定了基础。
Objective: To prepare PP1 liposomes and screen the optimal prescription. Methods: PP1 liposomes were prepared by membrane hydration method. The entrapment efficiency of PP1 liposomes was determined by high performance liquid chromatography (HPLC). The entrapment efficiency was the main index. Lipid ratio, cholesterol phospholipid ratio, temperature And hydration time as a factor, using orthogonal test to screen the optimal formula. Results: The optimum formulation of PP1 liposome prepared by the membrane water method was as follows: the lipid-lipid ratio was 1:10, the ratio of cholesterol to dipalmitoylphosphatidylcholine (DPPC) was 1: 8, the temperature was 40 ℃ , The hydration time is 3 min. The average entrapment efficiency was (63.27 ± 3.32)%. The mean diameter of PP1 liposomes was (157.73 ± 9.74) nm and the Zeta potential was (-4.74 ± 0.44) mV. CONCLUSION: PP1 liposome prepared by membrane hydration method has high encapsulation efficiency, uniform morphology and particle size and good reproducibility, which lays the foundation for the study of its sustained release in the eye.