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采用树皮下接种和打孔接种的方法,在板栗树苗和离体栗枝上测定了栗疫病菌的致病力。结果表明,两种接种方法测定病菌致病力比较一致;据此可将菌株分为强致病力和低致病力两类。对低致病力菌株分生孢子后代的不同培养型也进行了致病力测定,野生型单孢菌株的毒力明显比亲本型单孢菌株的强。讨论了低致病力菌株的毒力降低的原因。
Pathogenicity of Cryphonecoccus crisceae was determined on chestnut seedlings and detached chestnut branches by inoculation and puncture inoculation. The results showed that the pathogenicity of the two inoculation methods was consistent, and the strains were divided into two groups, which were strong virulence and low pathogenicity. The virulence of wild-type single-spore strain was significantly higher than that of the parental single-spore strain. The reasons for the reduced virulence of low pathogenic strains are discussed.