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采用聚合酶链反应-限制性片段长度多态性（PCR－RFLP）技术检测50例动脉硬化性脑梗死（ABI）患者和113例正常人戴脂蛋白E（apoE）基因型，并分析apoE基因多态。队与血脂脂蛋白和载脂蛋白之间的关系。结果表明：ABI组ε4/3基因型与ε4等位基因频率分别为30．0％与18．0％，均显著高于正常对照组的11．5％与7.5％（P＜0．01）。apoE基因多态性明显影响血脂水平，不同基因型组间血清总胆固醇（TC）、低密度脂蛋白胆固醇（LDL－C）和载脂蛋白B（apoB）水平有显著性差异，ε4／3组上述3项指标均显著高于ε3／3、ε3／2组。各组间血清甘油三酯（TG）、高密度脂蛋白胆固醇（HDL－C）和载脂蛋白AI（apoAI）水平无明显差异。多元逐步回归与相关分析均证实，ε4等位基因与TC、LDL－C和apoB呈显著正相关。上述结果提示，apoE基因多态性与ABI之间存在着密切关系，ε4等位基因可作为我国汉族人群ABI易感个体的一种遗传标记。
50 cases of atherosclerotic cerebral infarction (ABI) and 113 normal subjects were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the apoE gene Polymorphism. The relationship between the team and the blood lipoproteins and apolipoproteins. The results showed that the frequency of ε4 / 3 genotype and ε4 allele in ABI group were 30.0% and 18.0%, respectively, which were significantly higher than those in control group (11.5% vs 7.5%, P <0.01) . The level of apoE gene polymorphism significantly affected the level of serum lipids. There were significant differences in the levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and apolipoprotein B (apoB) between different genotype groups The above three indicators were significantly higher than the ε3 / 3, ε3 / 2 group. There were no significant differences in serum triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and apolipoprotein AI (apoAI) between the groups. Multiple stepwise regression and correlation analysis confirmed that the ε4 allele was significantly and positively correlated with TC, LDL-C and apoB. These results suggest that there is a close relationship between apoE gene polymorphism and ABI, ε4 allele can be used as a Han Chinese ABI susceptible individuals a genetic marker.