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目的:检测食管癌组织及其区域淋巴结中树突状淋细胞(DC)及Th1/Th2细胞因子的表达,探讨食管癌发生及与免疫逃逸的关系。方法:采用流式细胞术检测57例食管癌组织、癌旁组织,16例食管炎性组织,62枚区域淋巴结中DCCD1a、CD83及共刺激因子CD80、CD86的表达,ELISA法检测组织匀浆中Th1型细胞因子IL-2、IFN-γ,Th2型细胞因子IL-4、IL-10的水平。结果:57例食管癌组织中CD1A、CD83、CD80及CD86的表达分别为7.34±1.59、2.87±0.55、5.75±1.06和27.34±7.21,显著低于炎性、正常和癌旁组织;癌组织中IL-2、IFN-γ表达水平分别为8.94±1.72、9.34±2.16,低于炎性、正常和癌旁组织;IL-4、IL-10的水平分别为5.24±0.35、5.3±1.02,高于炎性、正常和癌旁组织(P<0.05,P<0.01)。区域淋巴结组织中CD1A、CD83、CD80及CD86的表达在有转移癌者明显低于无转移癌者;IL-2、IFN-γ表达水平降低;IL-4、IL-10的水平增高,差异有统计学意义(P<0.05,P<0.01)。结论:食管肿瘤组织和区域淋巴结内DC的减少及Th1/Th2失调,可能是食管肿瘤发生免疫逃逸的原因。
Objective: To detect the expression of dendritic lymphocytes (DCs) and Th1 / Th2 cytokines in esophageal cancer tissues and their regional lymph nodes, and to explore the relationship between esophageal carcinogenesis and immune escape. Methods: Flow cytometry was used to detect the expression of DCCD1a, CD83 and costimulatory molecules CD80 and CD86 in 57 cases of esophageal cancer tissues, 16 cases of esophageal inflammatory tissues and 62 cases of regional lymph nodes. Th1 type cytokines IL-2, IFN-γ, Th2 type cytokines IL-4, IL-10 levels. Results: The expressions of CD1A, CD83, CD80 and CD86 in 57 cases of esophageal carcinoma were 7.34 ± 1.59, 2.87 ± 0.55, 5.75 ± 1.06 and 27.34 ± 7.21, respectively, which were significantly lower than those in inflammatory, normal and paracancerous tissues The levels of IL-2 and IFN-γ were 8.94 ± 1.72 and 9.34 ± 2.16, respectively, which were lower than those in inflammatory, normal and paracancer tissues. The levels of IL-4 and IL-10 were 5.24 ± 0.35 and 5.3 ± 1.02 respectively In inflammatory, normal and paracancerous tissues (P <0.05, P <0.01). The expression of CD1A, CD83, CD80 and CD86 in regional lymph nodes was significantly lower in patients with metastasis than in those without metastasis; the expression of IL-2 and IFN-γ were decreased; the levels of IL-4 and IL-10 were increased Statistical significance (P <0.05, P <0.01). Conclusion: The decrease of DC and the imbalance of Th1 / Th2 in esophageal tumor tissues and regional lymph nodes may be the reason of esophageal tumor immune escape.