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目的:通过应用纳洛酮干预后,观察DBI合并SBI后血浆β-EP与c-fos基因表达的变化,进一步探讨血浆β-EP与c-fos基因在SBI中的作用。方法:70只雄性SD大鼠随机分为DBI合并SBI组(A组),DBI合并SBI后盐酸纳洛酮治疗组(B组),DBI后SBI前盐酸纳洛酮治疗组(C组);分别采用免疫组化及放免分析测定大鼠脑内c-fos蛋白与血浆β-EP的含量,并进行脑组织病理显微结构的观察。结果:B、C两组在各时间点的各项检测指标之间,均无显著性差异。B、C两组脑组织含水量在3h及24h时,均较A组明显升高(P<0.05);神经元损伤数量在24h及48h均较A组明显减少(P<0.05);伤后3h、24h及48h,B、C两组的c-fos基因表达均明显高于A组;伤后3h、24h,B、C两组的血浆β-EP含量也明显高于A组(P<0.05)。结论:盐酸纳洛酮可降低DBI合并SBI大鼠c-fos基因表达及血浆β-EP含量,降低死亡率及神经损伤程度,具有一定神经保护作用;DBI合并SBI后使用盐酸纳洛酮与DBI后预处理使用盐酸纳洛酮对SBI的疗效相仿。
OBJECTIVE: To observe the changes of plasma β-EP and c-fos gene expression after the intervention of naloxone in SBI and SBI, and further explore the role of plasma β-EP and c-fos in SBI. Methods: Seventy male Sprague - Dawley rats were randomly divided into group DBI with SBI (group A), DBI with naloxone hydrochloride (group B) after SBI, group with naloxone hydrochloride before SBI after DBI (group C). Immunohistochemistry and radioimmunoassay were used to determine the content of c-fos protein and plasma β-EP in rat brain, and the histopathological microstructure was observed. Results: There was no significant difference between the detection indexes of B and C groups at all time points. Compared with group A, the water content of brain tissue in groups B and C were significantly increased at 3 h and 24 h (P <0.05), and the number of neuronal damage was significantly decreased at 24 and 48 h (P <0.05) At 3h, 24h and 48h, the expression of c-fos gene in B and C groups was significantly higher than that in A group. The levels of plasma β-EP in B and C groups were also significantly higher than those in A group at 3h, 24h after injury 0.05). Conclusion: Naloxone hydrochloride can reduce the expression of c-fos gene and the content of plasma β-EP in DBI with SBI, reduce the mortality and the degree of nerve injury, and have neuroprotective effect. Naloxone hydrochloride and DBI Postconditioning using naloxone hydrochloride has similar effects on SBI.