防止组织培养外植体接种后褐化的方法

• 作 者: 赵玉军
• 出 处: 山西果树
• 发表日期: 1995年02期
• 关键词: 组织培养 褐化 短枝型 苹果品种 植物组织 物质氧化 赵玉 无菌水

防止组织培养外植体接种后褐化的方法组织培养外植体初始接种遇到最严重的问题是伤口酚类物质氧化变褐，而酚类物质及其氧化物对植物组织有害，因此必须去除。试用了四个苹果品种：ＧｒａｎｎｙＳｍｉｔｈ、Ｊｕｌｙｒｅｄ、Ｍｕｔｓｕ及短枝型金冠。研究表明下列方法可有...

Preventing tissue culture explants from browning after inoculation Tissue culture explants The most serious problem with initial inoculation of the explants is the oxidation and browning of the phenolic material in the wound, whereas the phenolic substances and their oxides are detrimental to plant tissues and must therefore be removed . Four varieties of apples were tried: Granny Smith, Julyred, Mutsu, and Spur Crown. Research shows that the following methods may have ...