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小鼠皮下免疫、腹腔攻击模型作为验证减毒痢疾活菌苗效力的指标已沿用多年 ,但其能否代表痢疾活菌苗的效力还有待商榷。实验选用口服痢疾活菌苗FS ,以不同活菌含量来验证该模型的意义。实验表明 ,菌体皮下免疫均能产生类似的保护效果 ,与活菌含量无关 ,血清学试验也论证了同样观点 ,而人体试验表明 ,菌苗的保护效果与活菌数密切相关 ,故认为小鼠皮下免疫、腹腔攻击模型不能准确反应痢疾菌苗的保护效果。
The subcutaneous immunization and intraperitoneal challenge models of mouse have been used for many years to verify the efficacy of live attenuated vaccine. However, whether they can represent the efficacy of live dysentery vaccine remains to be discussed. The experiment selected oral dysentery live vaccine FS, with different live bacteria content to verify the significance of the model. Experiments show that the subcutaneous immune cells can produce a similar protective effect, and has nothing to do with the content of live bacteria, serological tests also demonstrate the same point of view, and human tests show that the protective effect of the vaccine is closely related with viable count, it is considered small Subcutaneous immunization, intraperitoneal challenge model can not accurately reflect the protective effect of dysentery vaccine.