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A new experimental calibration was undertaken in this study to get a more reliable sphalerite-galenasulfur isotope geothermometer. The experimental conditions selected in study were very similar to those of naturalhydrothermal solution. The high-precision SF6 method was used in sulfur isotope analyses. The obtained calibrationcurve for sulfur isotope fractionation between sphalerite and galena can be expressed with the equation 1000lnαSp-Gn=0.74×106T-2+0.08.