论文部分内容阅读
紫河车,出自《本草纲目》。又名混沌衣、人胞。有补气、养血、温肾、益精之功效。近年来对胎盘及其制剂的研究已日益受到重视。本文在观察了胎盘因子(PF)对T淋巴细胞的增殖影响的基础上,应用CTLL细胞株作为指示细胞以及碱性磷酸酶-抗碱性磷酸酶(APAAP)免疫组化染色对诱导的外周血单个核细胞(PBMC)IL-2产生以及IL-2R的表达进行了研究。材料与方法一、胎盘因子制备:按文献进行。二、人外周血单个核细胞分离:取正常献血者新
Placenta, from the “Compendium of Materia Medica”. Also known as chaos clothing, human cells. There are qi, nourishing, warming kidney, Yi Jing effect. In recent years, the study of placenta and its preparation has been increasingly valued. In this paper, based on the observation of the effect of placental factor (PF) on the proliferation of T lymphocytes, CTLL cell line was used as indicator cells and alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemical staining was used to induce peripheral blood. Mononuclear cells (PBMC) IL-2 production and IL-2R expression were studied. Materials and Methods 1. Placental factor preparation: According to literature. Second, the separation of human peripheral blood mononuclear cells: take normal blood donors new