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目的了解台州市食品中分离的沙门菌耐药性、毒力因子以及基因分型情况,建立食源性沙门菌的分子特征本底信息,为食源性疾病的防治提供技术支撑。方法对近几年从食品中分离的22株沙门菌进行12种抗生素药敏试验、10种毒力基因PCR检测、脉冲电场凝胶电泳(PFGE)基因分型,用BioNumerics 5.0软件对分型数据进行聚类分析。结果 22株食源性沙门菌的总耐药率为59.1%,耐药率居前三位的抗生素分别是复方新诺明(36%)、四环素(27%)、萘啶酸(27%);所有菌株均检出6种以上毒力因子,1株肠炎沙门菌存在毒力岛、质粒及噬菌体等多种毒力因子;PFGE分型共得到21个条带,可分为5个基因型别,包括18种指纹图谱,各基因型别间同源性小于70%。结论台州市食源性沙门菌存在致病风险,建立的指纹图谱数据库可为食源性疾病的防治提供技术支持。
Objective To understand the drug resistance, virulence factors and genotyping of Salmonella isolated from foodstuffs in Taizhou and establish the molecular background information of foodborne Salmonella to provide technical support for the prevention and treatment of foodborne diseases. Methods Twenty-two antibiotics susceptibility tests, 10 virulence genes PCR and PFGE genotyping were performed on 22 strains of Salmonella isolated from food in recent years. BioNumerics 5.0 software was used to analyze the typing data Cluster analysis. Results The total drug resistance of 22 food-borne Salmonella strains was 59.1%. The top three antibiotics were cotrimoxazole (36%), tetracycline (27%), nalidixic acid (27%), ; All strains were detected more than 6 virulence factors, a S. enteritidis virulence islands, plasmids and phage and other virulence factors; PFGE typing a total of 21 bands can be divided into five genotypes Do not, including 18 kinds of fingerprints, homology between the genotypes less than 70%. Conclusion The risk of food-borne Salmonella in Taizhou is high. The established fingerprint database can provide technical support for the prevention and treatment of food-borne diseases.