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1987年9月我们用家兔经被膜穿刺法注入鱼肝油酸钠,观察注射后机体的反应,以及脾脏的转归,实验取得了阶段性结果,初步报道于后。 1 材料与方法 取1kg～1.1kg家兔20只,雌雄不限。分别采耳缘血查血色素、白细胞、血小板,随机分为四组,正常食水,分笼喂养。采用剖脾腹脏注射法,用3％的戊巴比妥钠溶液3ml～4ml/kg体重,静脉注射麻醉后,在左肋缘下约1cm处沿肋弓方向作长3cm切口,切开腹壁,暴露脾脏,分别在脾脏的中下2/3,经脾被膜穿刺注入无菌鱼肝油酸钠0.5ml左右观察10min后关腹。 第一组于注射后1周处死,第二、第三、四组分
In September, 1987, we injected sodium morronisal sodium into the rabbit through the capsule puncture and observed the response of the body after the injection and the outcome of the spleen. The preliminary results were obtained after the preliminary report. 1 Materials and methods Take 1kg ~ 1.1kg rabbits 20, male or female. Blood were collected from the otolaryngology hemoglobin, white blood cells, platelets were randomly divided into four groups, normal water, cage feeding. Using spleen abdominal injection, with 3% sodium pentobarbital solution 3ml ~ 4ml / kg body weight, intravenous anesthesia, about 1cm below the left costal margin along the direction of the rib arch for 3cm incision, incision abdominal wall , Exposed spleen, respectively, in the spleen 2/3, puncture the spleen capsule into the sterile sodium morronisallate 0.5ml observed 10min after the abdomen. The first group was sacrificed one week after injection, and the second, third and fourth groups