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采用血凝技术检测棉花各阶段各组织的浸提液和棉花种子分离的蛋白质组分的血凝活性, 並进行枯萎病菌培养试验, 结果发现: (1)棉花品种抗枯萎病能力与其种子血凝活性呈正相关;(2)抗枯萎病的棉花品种血凝活性主要表现在以下各个部位:种子的于叶, 种子萌发阶段的胚根, 棉苗生长阶段的根、 茎的韧皮部、 叶片以及授粉后种子形成的第45 55天直至成熟; (3)抗病品种种子浸提液及其分离的蛋白质组分能显著抑制枯萎病菌生长; (4)在一定范围内, 较高(50-100g/0.1mz)的枯萎病菌菌量可能是棉花抗枯萎病基因充分表达的重要诱导因素, 棉花凝集素的合成晋棉花枯茬病菌的调拧.
The hemagglutination test was used to detect the hemagglutination activity of the protein fractions extracted from the tissues of cotton and the seed of cotton from different stages of cotton. The results showed that: (1) (2) The hemagglutination activity of cotton cultivars resistant to Fusarium wilt mainly manifested in the following parts: seed in leaves, seed germination stage radicle, cotton seedling growth stage roots, stem phloem, leaves and after pollination Seed formation 45th day until maturity; (3) resistant variety seed extract and its isolated protein components can significantly inhibit the growth of Fusarium wilt; (4) in a certain range, higher (50-100g / 0.1 The amount of Fusarium oxysporum fumigatus could be an important inducing factor for the full expression of Fusarium wilt resistance genes in cotton.