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目的考察PELGE空白纳米粒对Chang细胞原癌基因、抑癌基因表达量的影响,筛选生物相容性好、低毒的载体材料,探索纳米粒可能存在的致癌性。方法采用实时荧光定量PCR(Real-time PCR)技术,用荧光标记寡聚核苷酸,Tagman探针法测定与PLEGE和PLGA空白纳米粒共培养的Chang氏细胞,以管家基因β-actin为内参基因,测定了抑癌基因p53及原癌基因c-myc、c-fos表达量的变化。结果 9种空白PELGE纳米粒中,1号材料(PEG 550,5%,LA/GA=7:3)、7号材料(PEG 750,5%,LA/GA=7:3)的3种基因表达水平均较低,相对于空白对照无显著差异。结论 1、7号材料性质惰性,不会导致细胞出现原癌基因、抑癌基因表达量的明显变化,说明其生物相容性良好,无潜在的致癌性。
Objective To investigate the effect of PELGE blank nanoparticles on the expression of proto-oncogenes and tumor suppressor genes in Chang cells and screen the carrier material with good biocompatibility and low toxicity to explore the possible carcinogenicity of nanoparticles. Methods Real-time quantitative PCR (Real-time PCR) was used to detect Chang’s cells co-cultured with PLEGE and PLGA blank nanoparticles with fluorescent labeled oligonucleotide and Tagman probe. Gene, determination of tumor suppressor gene p53 and proto-oncogene c-myc, c-fos expression changes. Results Among the 9 kinds of blank PELGE nanoparticles, three kinds of genes (PEG 550, 5%, LA / GA = 7: 3) and No.7 (PEG 750,5%, LA / GA = 7: 3) The expression levels were lower than the blank control, no significant difference. Conclusions No. 1 and No. 1 are inert in nature and do not cause significant changes in the expression of protooncogenes and tumor suppressor genes in cells, indicating that they have good biocompatibility and no potential carcinogenicity.