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作者用Percoll氏梯度法收集活动的人精子,并用人血清、各种血清成分、滤泡液及精浆评价其对精子活动力的作用。在单纯培养介质(RPMI-1640)中精子活动力在5小时后丧失,加入男子血清可明显加强精子活动力及延长存活率。白蛋白、精浆及滤泡液也都能刺激精子活动力但作用较血清小。男子血清与女子血清未见差别,不论女子血清采自激素刺激周期的滤泡期或黄体期,或是含
The authors used Percoll’s gradient method to collect active human sperm and evaluated their effect on sperm motility using human serum, various serum components, follicular fluid and seminal plasma. In pure culture medium (RPMI-1640) sperm motility in 5 hours after the loss, adding male serum can significantly enhance sperm motility and prolong survival. Albumin, seminal plasma and follicular fluid can also stimulate sperm motility, but the role of less than serum. Men’s serum and women’s serum no difference, regardless of female serum collected from the hormone cycle of the follicular or luteal phase, or containing