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目的了解北京市朝阳区蚊虫携带辽宁病毒(LNV)的情况。方法按照《病媒生物密度监测方法蚊虫》(GB/T 23797-2009)中的方法,于2014-2015年采用CO2诱蚊灯采集蚊虫,利用反转录聚合酶链反应检测LNV核酸,采用Mega 6.0软件进行进化分析。结果2014—2015年共采集蚊虫9811只,其中淡色库蚊为优势种,占捕获总数的91.41%(8968/9811)。标本编号BJCY14007的淡色库蚊核酸经检测为LNV阳性。基于LNV第10节段的分子特征显示,LNV分支为两个进化群血清Ⅰ型和血清Ⅱ型,BJCY14007与NE97-12较为接近,属血清Ⅰ型。结论北京市首次报道从蚊虫分离出LNV。因LNV有潜在的致病性,应注意其分布及变化情况,且有必要在人群及宿主动物中开展血清学调查。
Objective To understand the mosquito-borne Liaoning virus (LNV) in Chaoyang District of Beijing. Methods The mosquitoes were collected by CO2 trapping lamp from 2014 to 2015 in accordance with the method of “vector for monitoring the density of vector organisms” (GB / T 23797-2009). The nucleic acid of LNV was detected by reverse transcription polymerase chain reaction 6.0 software for evolutionary analysis. Results A total of 9811 mosquitoes were collected from 2014 to 2015, of which Culex pipiens pallens were the dominant species, accounting for 91.41% (8968/9811) of the total catch. The Culex pipiens pallens nucleic acid of the specimen number BJCY14007 was tested positive for LNV. Based on the molecular characteristics of the tenth segment of LNV, LNV was divided into two evolutionary serogroups I and II, and BJCY14007 and NE97-12 were more closely related to serotype I. Conclusion Beijing was the first to report the isolation of LNV from mosquitoes. Due to the potential pathogenicity of LNV, attention should be paid to its distribution and changes, and there is a need to conduct serological surveys in populations and host animals.